Bd Facs Canto Ii User Manual
- Bd Facs Canto Ii Manual
- Bd Facscanto Ii Instructions For Use
- Bd Canto Ii Manual
- Bd Facscanto Ii User Guide
- Bd Facscanto Ii Manual
- Bd Canto Ii Compensation Instructions
BD FACSVia ™ flow cytometry system. The BD FACSVia flow cytometry system is an easy-to-use benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement, labor-intensive manual procedures, fewer resources and higher demands for efficiency, so your laboratory runs smoothly and efficiently. BD FACSCanto II Operation Manual 1 FACSCanto II Standard Operation Protocol Basic Operation 1. Make sure the following steps are done before running your samples. Waste tank is empty and refill the bottom layer of waste tank with bleach. There is enough amount of sheath fluid to perform machine start up and sample acquisition. Page 1 BD FACSCanto II Flow Cytometer Quick Reference Guide This guide contains instructions for both BD FACSCanto™ clinical and BD FACSDiva™ software. Follow only those sections that apply to the software you are running. Starting Up Turn on the cytometer main power. Page 2 Daily Setup: BD FACSCanto Clinical Software Set up the cytometer with BD FACSCanto clinical software, even if you. Blizard Institute Flow Cytometry Core Facility FACS Canto II Quick User Guide Page 5 1.2 FACS Canto II Shut Down Procedure 1. From the drop down menu under CYTOMETER, select the “Fluidics Shutdown” option. Press “OK” when prompted.This should take about 5 minutes.
14 BD FACSCanto II Instructions for Use System Components and Theory of Operation The BD FACSCanto II system consists of a flow cytometer, a self-contained fluidics cart, and the BD FACSCanto II workstation. System options include an automated sample loader and a barcode reader.
The BD FACSCanto™ II system features many innovations. At the heart of the cytometer, for example, the fluidics system features a fixed-alignment flow cell to minimize startup time and improve reproducibility. The BD FACSCanto II fluidics system is designed to streamline work, save time, and improve performance. In the fluidics system, the sample travels up the sample injection tube, and hydrodynamic focusing within the flow cell forces particles into a single-file stream where laser light intercepts the stream at the sample interrogation point. The unique flow cell design permits particles to flow through the center of the flow cell. Increasing the sample pressure increases the core diameter and the flow rate.
Lasers: Available in 2 (Blue 488, Red 633) and 3 (add Violet 405)
Detectors: Up to 10 parameters
Colors: Available in 6 and 8 color options
Footprint: 24” x 36” x 25”
DESCRIPTION
A proven platform for maximun reliability and the highest quality results built on more than 25 years of BD experience and leadership.A proven platform for maximum reliability and the highest quality results
Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high quality results. /panasonic-lumix-dmc-fz150-user-manual.html. The BD FACSCanto II provides the ultimate in flexibility and can be configured with two or three lasers to detect up to eight colors.
FORUMSView All (1)
Ask a New Question0Replies7 years ago | 7 years agoDO NOT GET THIS We purchased a Facs Canto II with a high through put attachment (HTS). The HTS broke down several times the first year of use, despite closely following the manufacturer's recommendations. The company kept sending us refurbished HTSs that were just as bad as the original part. After being tired of losing experiments we wanted to have a spare HTS to have so we could swap it out ourselves. The company's response? Charge us $8,100 dollars for the privilege of having a second subpar piece of equipment under a service contract, on top of the already overpriced service contract for the whole machine, which covers the original HTS. When this is older and we are ready to replace it we are definitely looking at BD competitors!!! Reply |
DOCUMENTS / MANUALSView All
FEATURES
High Performance, Innovative SystemThe BD FACSCanto™ II system features many innovations. At the heart of the cytometer, for example, the fluidics system features a fixed alignment flow cell to minimize startup time and improve reproducibility. The BD FACSCanto II fluidics system is designed to streamline work, save time, and improve performance. In the fluidics system, the sample travels up the sample injection tube, and hydrodynamic focusing within the flow cell forces particles into a single-file stream where laser light intercepts the stream at the sample interrogation point. The unique flow cell design permits particles to flow through the center of the flow cell. Increasing the sample pressure increases the core diameter and the flow rate.
Fluidics CartA fluidics cart holds large fluid tanks necessary to operate and maintain the instrument. For sample acquisition, positive-pressure pumps in the fluidics cart send sheath fluid past a 0.2-µm filter to a pressurized interior reservoir inside the instrument called the plenum. The plenum maintains a nearly constant fluid level and dampens pump pulsation using a new dynamic feedback pressure control system designed to regulate pressure. As a result, sheath flow rate does not vary with the level of fluid in the sheath cubitainer, and the reservoir automatically removes small air bubbles from the sheath supply.
Hydrodynamic focusingA high flow rate is generally used for measurements such as immunophenotyping, for which data is less resolved but is acquired more quickly. A lower flow rate is generally used in applications for which optimal resolution and sensitivity are critical.
SoftwareDaily routine procedures, such as startup, shutdown, and cleaning routine cycles, are automated as a result of fluidic integration with BD FACSCanto clinical software or BD FACSDiva™ software. BD FACS™ Shutdown solution prevents salt crystal buildup in fluidics lines and is supplemented with a preservative to prevent bacterial growth. During the instrument shutdown procedure, the BD FACS Shutdown solution replaces sheath fluid in all sample and sheath fluid lines.
Heska vet iv 2.2 infusion pump user manual. LED alertsLEDs are located in the front door to monitor each acquisition parameter. Each LED blinks when the signal level reaches a prescribed threshold.
Additional Specifications
Optics
Lasers
Air-cooled: 488-nm solid state, 20-mW laser output 633-nm HeNe, 17-mW laser output
Laser Configuration
Spatially separated beams with 9 x 65- µm elliptical spots
Optical Alignment Procedure
Fixed, no operator alignment required
Flow Cell
180 x 430-µm rectangular quartz flow cell
Collection Optics
Optical-gel coupled 1.2 NA lens
FSC Resolution
1.0 µm
SSC Resolution
0.5 µm
Fluorescence Detector Design
Reflective optics with single transmission filter in front of each PMT
FSC Detector
Photodiode with 488/10 BP
SSC Detector
PMT with 488/10 BP
Fluorescence Detectors
6 PMTs in 4-2 standard configuration
Blue Laser Dyes
Red Laser Dyes
APC, APC-Cy7 (660, 785 nm)
Blue Laser:530/30; 585/42; >670; 780/60 nm
Red Laser: 660/20; 780/60 nm
Fluorescence Threshold Sensitivities
FITC 15; PE >80; PerCP >9;
Filter Change Procedure
Keyed filters, no tools required
General Operation
Integrated fluidics cart and compressor with onboard housekeeping solutions for automated startup, shutdown, and cleaning cycles
Sheath Consumption
1.10 L/h normal operation; 670; 780/60 nm
Bd Facs Canto Ii Manual
Lasers
Air-cooled: 488-nm solid state, 20-mW output 633-nm HeNe, 17-mW output
Fluorescence Detectors
8 PMTs in 5-3 configuration
Laser Dyes
Detector Bands
Sample Input with BD FACS™ Loader Option Loading
40-tube carousel
Sample/test ID
Throughput
56 min/carousel with BD™ Multi-check high controls, 66 min/carousel with BD Multi-check low controls using BD Multitest™ 6-color TBNK application
Miscellaneous
Multiple clinical applications can be run on the same Loader carousel.
Sample Input with BD High Throughput Sampler Option**
Loading 96- and 384-well microtiter plates
Throughput
<15 min/96-well plate in high-through- put mode with 2-second acquisition
Carryover
=1%
Bd Facscanto Ii Instructions For Use
Barcode Reader with Stand Use with BD FACSCanto clinical software2D Reader
Streamlined input of BD FACS™ 7-color setup bead target values, input of patient information
Bd Canto Ii Manual
Installation Requirements
Bd Facscanto Ii User Guide
Weight
Bd Facscanto Ii Manual
Bd Canto Ii Compensation Instructions
Power
100/115/230 VAC, 50–60Hz
Operating Environment
16–30°C, 20–80% noncondensing relative humidity
Heat Dissipation with BD FACS Loader Installed
1,843 BTU/h